불화규산의 mutans streptococci에 대한 항균작용 및 인체돌연변이 유발가능성에 관한 연구
Antibacterial effect on mutans streptococci and mutagenecity of fluorosilicic acid
박은아, 박근영, 박병래, 김유영, 윤현경, 최기운, 이진용,
소속 상세정보
박은아 ( ) - 경희대학교 치과대학 구강생물학연구소
박근영 ( ) - 경희대학교 치과대학 구강생물학연구소
박병래 ( ) - 경희대학교 치과대학 구강생물학연구소
김유영 ( ) - 경희대학교 치과대학 구강생물학연구소
윤현경 ( ) - 경희대학교 치과대학 구강생물학연구소
최기운 ( ) - 경희대학교 치과대학 구강생물학연구소
이진용 ( ) - 경희대학교 치과대학 구강생물학연구소
KMID : 0355420010250040415
Abstract
Antibacterial effect of F at lower concentrations present in fluoridated water and local application of F on cariogenic bacteria is controversial. The present study was performed to examine antibacterial effect of fluorosilicic acid at various concentrations and long-term effect of the fluoride at lower concentrations on representative strains of mutans streptococci: S. mutans NCTC 10449 and S. sobrinus 6715. For the purpose, physico-chemical characteristics of the bacteria treated with fluorosilicic acid were observed.
Minimum inhibitory concentration (MIC) of F- for S. mutans and S sobrinus was determined to be 50 and 100 ppm, respectively, as measured by optical density. The addition of F at MIC to mutans streptococci in the exponential phase decreased the cell viability by more than 80%. F in combination with xylitol and polyphosphate slightly enhanced their antibacterial activity. The experimental strains exposed to F- (0-15 ppm) for 2 years on blood agar plates containing fourosilicic acid demonstrated to obtain resistance to some extent to F. F exposure decreased the inhibition of the bacterial growth by Fas observed after the incubation with F at the range of MIC, the concentration determined 2 years ago. The decrease in the inhibition of growth and survival by F was more prominent with the strains long-term exposed to higher concentrations of F- . The pH of the F- -exposed strains when cultured in broth containing fluorosilicic acid was significantly lower than that of the strains which had not been exposed to F. Difference in pH between 4- and 24-h incubation was more prominent with the strains long-term exposed to F, suggest that F exposure conferred F resistance to the mutans streptococci. The higher concentrations of F the strains exposed to, the higher synthesis of soluble glucan was measured in S. mutans, resulting in higher binding of the bacterium to glass surface. However, those were decreased in S. sobrinus long-term exposed to higher concentrations of F. Regardless whether the bacteria were exposed
to F or not, no significant difference in binding to saliva-coated hydroxyapatite and protein profiles. Transmission electron micrographs revealed that loss of the cell wall and vacuolization in the cytoplasm were distinct in the bacteria exposed to higher concentrations of F. It was shown in Ames test that F- up to 100 ppm appeared to be negative for mutagenicity.
Overall, the results suggest that combination of fluorosilicic acid with other anticariogenic reagents may be benefical for a better caries prevention. Use of fluoride for long-term period of time even at lower concentrations may contribute to the efficacy of fluoride in caries prevention, inhibiting the bacterial function and metabolism,
키워드
불화규산;mutans streptococci;항세균효과;돌연변이
원문 및 링크아웃 정보
등재저널 정보